Block Type 96-Well 0.1-mL Block
Calibration Background is expired No
Calibration Background performed on 09-23-2021
Calibration Pure Dye ABY is expired No
Calibration Pure Dye ABY performed on 09-23-2021
Calibration Pure Dye CY5 is expired No
Calibration Pure Dye CY5 performed on 09-23-2021
Calibration Pure Dye FAM is expired No
Calibration Pure Dye FAM performed on 09-23-2021
Calibration Pure Dye JUN is expired No
Calibration Pure Dye JUN performed on 09-23-2021
Calibration Pure Dye MUSTANG PURPLE is expired No
Calibration Pure Dye MUSTANG PURPLE performed on 09-23-2021
Calibration Pure Dye NED is expired No
Calibration Pure Dye NED performed on 09-23-2021
Calibration Pure Dye ROX is expired No
Calibration Pure Dye ROX performed on 09-23-2021
Calibration Pure Dye SYBR is expired No
Calibration Pure Dye SYBR performed on 09-23-2021
Calibration Pure Dye TAMRA is expired No
Calibration Pure Dye TAMRA performed on 09-23-2021
Calibration Pure Dye VIC is expired No
Calibration Pure Dye VIC performed on 09-23-2021
Calibration ROI is expired No
Calibration ROI performed on 09-23-2021
Calibration Uniformity is expired No
Calibration Uniformity performed on 09-23-2021
Chemistry SYBR_GREEN
Date Created 2022-08-24 13:05:11 PM SGT
Experiment Barcode NA
Experiment Comment NA
Experiment File Name D:BiosystemsDesign & Analysis SoftwareFilesCell HEK293 DNA\2022-08-23_PBMC.eds
Experiment Name 2022-08-23_132729
Experiment Run End Time 2022-08-23 15:14:47 PM SGT
Experiment Type Standard Curve
Instrument Name 272511079
Instrument Serial Number 272511079
Instrument Type QuantStudio™ 5 System
Passive Reference ROX
Post-read Stage/Step NA
Pre-read Stage/Step NA
Quantification Cycle Method Ct
Signal Smoothing On true
Stage where Melt Analysis is performed Stage3
Stage/ Cycle where Ct Analysis is performed Stage2, Step2
User Name Deborah Chen

Assay Controls:

Summary Assay controls are valid.
Negative Control Assay controls are valid.
Positive Control DPC amplification successful.
Inhibition Control Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.

1. Negative Control (NTC)

No unspecific amplification for NTC.

Well Position name type Sample Name CT Tm Melt Peak Height Results
H1 Negative NEG Negative 38.25 74.82 53430.22 Negative Control Pass

2. Positive Control

DPC amplification successful.

Well Position name type Sample Name CT Tm Melt Peak Height Results
H12 Positive POS Positive 25.6 84.8 267908 Positive Control Pass

IHC Controls

Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.

Well Position name type Sample Name CT Tm Melt Peak Height Results
A8 U01 IHC U01_IHC 25.16 84.94 239034.9 REVIEW: DPC Detected
C8 U02 IHC U02_IHC 25.11 84.81 261770.4 REVIEW: DPC Detected
E8 U03 IHC U03_IHC 25.17 84.81 230682.6 REVIEW: DPC Detected
G8 U04 IHC U04_IHC 25.12 84.81 258255.5 REVIEW: DPC Detected
G8 U04 IHC U04_IHC 25.12 77.26 133946.0 REVIEW: Failed IHC prevented call

4. Results

Samples that failed IHC are indicated in red.

CT for Unknown Samples

Well Position name type Sample Name CT Ct Mean Ct SD
A3 U01 UNK U01 32.40 32.15 0.39
A4 U01 UNK U01 31.70 32.15 0.39
A5 U01 UNK U01 32.35 32.15 0.39
C3 U02 UNK U02 31.29 31.37 0.27
C4 U02 UNK U02 31.16 31.37 0.27
C5 U02 UNK U02 31.67 31.37 0.27
E3 U03 UNK U03 31.79 31.68 0.20
E4 U03 UNK U03 31.46 31.68 0.20
E5 U03 UNK U03 31.81 31.68 0.20
G3 U04 UNK U04 32.14 32.25 0.13
G4 U04 UNK U04 32.21 32.25 0.13
G5 U04 UNK U04 32.39 32.25 0.13

Tm for Unknown Samples

Well Position name type Sample Name Tm Melt Peak Height
A3 U01 UNK U01 85.29 136974.06
A3 U01 UNK U01 78.16 85896.86
A3 U01 UNK U01 74.40 84885.14
A4 U01 UNK U01 85.29 158222.12
A4 U01 UNK U01 74.40 93612.84
A5 U01 UNK U01 85.20 140391.53
A5 U01 UNK U01 74.82 140192.53
A5 U01 UNK U01 65.53 66223.98
C3 U02 UNK U02 74.80 146811.38
C3 U02 UNK U02 85.16 113407.70
C4 U02 UNK U02 75.07 160499.08
C4 U02 UNK U02 85.16 97340.31
C5 U02 UNK U02 77.52 148725.30
C5 U02 UNK U02 85.06 97270.88
E3 U03 UNK U03 85.16 144826.98
E3 U03 UNK U03 77.62 167174.16
E4 U03 UNK U03 85.02 134525.97
E4 U03 UNK U03 77.62 164710.42
E5 U03 UNK U03 84.79 128606.00
E5 U03 UNK U03 74.96 139405.31
G3 U04 UNK U04 77.76 215730.16
G3 U04 UNK U04 85.16 127013.79
G4 U04 UNK U04 85.16 113766.78
G4 U04 UNK U04 74.93 136980.22
G5 U04 UNK U04 84.79 105212.19
G5 U04 UNK U04 77.65 117258.88
G5 U04 UNK U04 74.42 116117.14
End of Report