| Block Type | 96-Well 0.1-mL Block |
| Calibration Background is expired | No |
| Calibration Background performed on | 09-23-2021 |
| Calibration Pure Dye ABY is expired | No |
| Calibration Pure Dye ABY performed on | 09-23-2021 |
| Calibration Pure Dye CY5 is expired | No |
| Calibration Pure Dye CY5 performed on | 09-23-2021 |
| Calibration Pure Dye FAM is expired | No |
| Calibration Pure Dye FAM performed on | 09-23-2021 |
| Calibration Pure Dye JUN is expired | No |
| Calibration Pure Dye JUN performed on | 09-23-2021 |
| Calibration Pure Dye MUSTANG PURPLE is expired | No |
| Calibration Pure Dye MUSTANG PURPLE performed on | 09-23-2021 |
| Calibration Pure Dye NED is expired | No |
| Calibration Pure Dye NED performed on | 09-23-2021 |
| Calibration Pure Dye ROX is expired | No |
| Calibration Pure Dye ROX performed on | 09-23-2021 |
| Calibration Pure Dye SYBR is expired | No |
| Calibration Pure Dye SYBR performed on | 09-23-2021 |
| Calibration Pure Dye TAMRA is expired | No |
| Calibration Pure Dye TAMRA performed on | 09-23-2021 |
| Calibration Pure Dye VIC is expired | No |
| Calibration Pure Dye VIC performed on | 09-23-2021 |
| Calibration ROI is expired | No |
| Calibration ROI performed on | 09-23-2021 |
| Calibration Uniformity is expired | No |
| Calibration Uniformity performed on | 09-23-2021 |
| Chemistry | SYBR_GREEN |
| Date Created | 2022-08-24 13:05:11 PM SGT |
| Experiment Barcode | NA |
| Experiment Comment | NA |
| Experiment File Name | D:BiosystemsDesign & Analysis SoftwareFilesCell HEK293 DNA\2022-08-23_PBMC.eds |
| Experiment Name | 2022-08-23_132729 |
| Experiment Run End Time | 2022-08-23 15:14:47 PM SGT |
| Experiment Type | Standard Curve |
| Instrument Name | 272511079 |
| Instrument Serial Number | 272511079 |
| Instrument Type | QuantStudioâ„¢ 5 System |
| Passive Reference | ROX |
| Post-read Stage/Step | NA |
| Pre-read Stage/Step | NA |
| Quantification Cycle Method | Ct |
| Signal Smoothing On | true |
| Stage where Melt Analysis is performed | Stage3 |
| Stage/ Cycle where Ct Analysis is performed | Stage2, Step2 |
| User Name | Deborah Chen |
| Summary | Assay controls are valid. |
| Negative Control | Assay controls are valid. |
| Positive Control | DPC amplification successful. |
| Inhibition Control | Warning: These samples ( U01, U02, U03, U04 ) had failed IHC. |
No unspecific amplification for NTC.
| Well Position | name | type | Sample Name | CT | Tm | Melt Peak Height | Results |
|---|---|---|---|---|---|---|---|
| H1 | Negative | NEG | Negative | 38.25 | 74.82 | 53430.22 | Negative Control Pass |
DPC amplification successful.
| Well Position | name | type | Sample Name | CT | Tm | Melt Peak Height | Results |
|---|---|---|---|---|---|---|---|
| H12 | Positive | POS | Positive | 25.6 | 84.8 | 267908 | Positive Control Pass |
Warning: These samples ( U01, U02, U03, U04 ) had failed IHC.
| Well Position | name | type | Sample Name | CT | Tm | Melt Peak Height | Results |
|---|---|---|---|---|---|---|---|
| A8 | U01 | IHC | U01_IHC | 25.16 | 84.94 | 239034.9 | REVIEW: DPC Detected |
| C8 | U02 | IHC | U02_IHC | 25.11 | 84.81 | 261770.4 | REVIEW: DPC Detected |
| E8 | U03 | IHC | U03_IHC | 25.17 | 84.81 | 230682.6 | REVIEW: DPC Detected |
| G8 | U04 | IHC | U04_IHC | 25.12 | 84.81 | 258255.5 | REVIEW: DPC Detected |
| G8 | U04 | IHC | U04_IHC | 25.12 | 77.26 | 133946.0 | REVIEW: Failed IHC prevented call |
Samples that failed IHC are indicated in red.
| Well Position | name | type | Sample Name | CT | Ct Mean | Ct SD |
|---|---|---|---|---|---|---|
| A3 | U01 | UNK | U01 | 32.40 | 32.15 | 0.39 |
| A4 | U01 | UNK | U01 | 31.70 | 32.15 | 0.39 |
| A5 | U01 | UNK | U01 | 32.35 | 32.15 | 0.39 |
| C3 | U02 | UNK | U02 | 31.29 | 31.37 | 0.27 |
| C4 | U02 | UNK | U02 | 31.16 | 31.37 | 0.27 |
| C5 | U02 | UNK | U02 | 31.67 | 31.37 | 0.27 |
| E3 | U03 | UNK | U03 | 31.79 | 31.68 | 0.20 |
| E4 | U03 | UNK | U03 | 31.46 | 31.68 | 0.20 |
| E5 | U03 | UNK | U03 | 31.81 | 31.68 | 0.20 |
| G3 | U04 | UNK | U04 | 32.14 | 32.25 | 0.13 |
| G4 | U04 | UNK | U04 | 32.21 | 32.25 | 0.13 |
| G5 | U04 | UNK | U04 | 32.39 | 32.25 | 0.13 |
| Well Position | name | type | Sample Name | Tm | Melt Peak Height |
|---|---|---|---|---|---|
| A3 | U01 | UNK | U01 | 85.29 | 136974.06 |
| A3 | U01 | UNK | U01 | 78.16 | 85896.86 |
| A3 | U01 | UNK | U01 | 74.40 | 84885.14 |
| A4 | U01 | UNK | U01 | 85.29 | 158222.12 |
| A4 | U01 | UNK | U01 | 74.40 | 93612.84 |
| A5 | U01 | UNK | U01 | 85.20 | 140391.53 |
| A5 | U01 | UNK | U01 | 74.82 | 140192.53 |
| A5 | U01 | UNK | U01 | 65.53 | 66223.98 |
| C3 | U02 | UNK | U02 | 74.80 | 146811.38 |
| C3 | U02 | UNK | U02 | 85.16 | 113407.70 |
| C4 | U02 | UNK | U02 | 75.07 | 160499.08 |
| C4 | U02 | UNK | U02 | 85.16 | 97340.31 |
| C5 | U02 | UNK | U02 | 77.52 | 148725.30 |
| C5 | U02 | UNK | U02 | 85.06 | 97270.88 |
| E3 | U03 | UNK | U03 | 85.16 | 144826.98 |
| E3 | U03 | UNK | U03 | 77.62 | 167174.16 |
| E4 | U03 | UNK | U03 | 85.02 | 134525.97 |
| E4 | U03 | UNK | U03 | 77.62 | 164710.42 |
| E5 | U03 | UNK | U03 | 84.79 | 128606.00 |
| E5 | U03 | UNK | U03 | 74.96 | 139405.31 |
| G3 | U04 | UNK | U04 | 77.76 | 215730.16 |
| G3 | U04 | UNK | U04 | 85.16 | 127013.79 |
| G4 | U04 | UNK | U04 | 85.16 | 113766.78 |
| G4 | U04 | UNK | U04 | 74.93 | 136980.22 |
| G5 | U04 | UNK | U04 | 84.79 | 105212.19 |
| G5 | U04 | UNK | U04 | 77.65 | 117258.88 |
| G5 | U04 | UNK | U04 | 74.42 | 116117.14 |